Original Research Article
Year: 2019 | Month: August | Volume: 9 | Issue: 8 | Pages: 29-34
Demethylation of RASSF1A Gene by Quercetin and Eugenol in HeLa Cancer Cell Line
Saloni1, Archita Sharma1, Harsh Goel1, Sweta Pal1, Pooja Rai1, Kavita Rawat2, Saima Syeda2, Anju Shrivastava2, Abhimanyu Kumar Jha11
1Department of Biotechnology, Institute of Applied Medicines and Research Ghaziabad, Uttar Pradesh, India
2Department of Zoology, Delhi University, India
Corresponding Author: Abhimanyu Kumar Jha
ABSTRACT
Cervical cancer is a potentially preventable disease and second most familiar malignancy among women worldwide with high incidence rates in developing regions. A worldwide approximated annual case of cervical cancer is 493,000 and ends with 273,500 every year deaths. In developing countries, it reports for about 85% of both its morbidity and fatality. Epigenetic silencing of neoplasm inhibitor genes may be reversed by numerous molecules additionally as natural compounds like polyphenols which are able to act as a demethylating agent. Quercetin and Eugenol have been found to specifically target numerous neoplasm inhibitor genes and modify their expression. To analyse the response of quercetin and eugenol on the methylation pattern of the RASSF1A gene in HeLa neoplastic cell line in a dose-dependent manner. The reversal of hypermethylation of the RASSF1A gene was observed after 6 days of treatment with 20 μM Quercetin and Eugenol in HeLa cells. To analyse, the reversal of methylation imitate of hypermethylated RASSF1A gene in HeLa cervical cancer cell line, it was treated with various concentrations of Quercetin and Eugenol for various time periods. Our results demonstrate that treatment of Cervical cells with Quercetin and Eugenol reversed the hypermethylation status of the RASSF1A gene.
Key words: Cervical cancer, HeLa cell line, Quercetin, Eugenol, Hypermethylation