Original Research Article
Year: 2019 | Month: May | Volume: 9 | Issue: 5 | Pages: 55-58
Phenotypic and Genotypic Characterisation of Vancomycin Resistant Enterococci (VRE) in a Tertiary Care Hospital of Kanpur, India
Khanal LK1, Bhatiani A2, Kumar A3
1PhD Scholar-Microbiology, 2Professor-Microbiology, 3Assistant Professor,
Central Research Laboratory; Rama Medical College Hospital & Research Centre, Kanpur-India.
Corresponding Author: Bhatiani A
ABSTRACT
Background: Vancomycin resistant enterococci related infection is an emerging nosocomial problem in India. This study was aimed to detect phenotypic and genotypic basis of VRE in a tertiary care hospital of northern India.
Methods: AST and high level gentamicin & high level streptomycin resistance pattern of enterococci isolates was determined by Kirby-Bauer’s disc diffusion method. VRE were detected by agar dilution method. Species differentiation was done according to Facklam & Collin’s classification. Molecular testing was done for detection of VanA & VanB genes among VRE.
Results: Out of 50 enterococci, 6(12%) were VRE. HLG & HLS resistance was 60% & 46% respectively. Species differentiation showed 16% isolates as E. faecium & 84% as E. faecalis. 5 out of 6 VRE were E. fecium. Molecular testing revealed, out of 6 VRE, 3 contained VanA or VanB genes and remaining 3 contained genes other than VanA or VanB responsible for vancomycin resistance.
Key words: VRE, HLG, HLS, VanA, VanB, hospital, Kanpur.