Original Research Article
Year: 2019 | Month: May | Volume: 9 | Issue: 5 | Pages: 46-54
Screening and Molecular Characterization of VISA and VRSA among the MRSA Isolates at a Tertiary Care Centre, Kanpur
Nashra A1, Sujatha R2, Deepak Sameer3, Anil Kumar4, Ajay Bagoliwal5
1Ph.D. Scholar, 2Professor and Head, 3Tutor, Department of Microbiology, 4Assistant Professor, Central Research Laboratory , 5Professor,
Department of Community medicine, Rama Medical College Hospital and Research Centre, Mandhana, Kanpur (UP) India.
Corresponding Author: Sujatha R
ABSTRACT
Background: Glycopeptides such as vancomycin are frequently the antibiotics of choice for the treatment of infections caused by methicillin resistant Staphylococcus aureus (MRSA). For the last few years incidence of vancomycin intermediate S. aureus and vancomycin resistant S. aureus (VISA and VRSA respectively) has been increasing in various parts of the world. The present study was carried out to find out the presence of VISA and VRSA among the MRSA isolates and its Molecular characterization at a tertiary care centre, Kanpur.
Objective: Screening and molecular characterization of VISA and VRSA among the MRSA isolates and its prevalence in Kanpur region.
Materials and Methods: Clinical samples were processed from hospitalized patients in Rama Medical College Hospital & Research Centre Mandhana, Kanpur. Out of 140 S. aureus isolates 40 were found MRSA by disk diffusion method, which were then confirmed as VISA and were VRSA by E-test, Vitek and MIC. Bacterial DNA was extracted and PCR for the detection of Mec A and Van A gene was performed.
Results: Out of 140 S. aureus isolates, the Prevalence of MRSA was 40 (28.57%) by disk diffusion method, in which 8 (5.7%) were confirmed as VISA and 2 (1.42%) were VRSA by E-test, Vitek and MIC. The molecular characterization of the test isolates detects the presence of Mec A gene, which was confirmed by sequencing but there was no Van A gene detected among these isolates.
Conclusion: The present study reveals the prevalence of MRSA/VISA and VRSA from the Kanpur region and indicates the magnitude of antibiotic resistance in and around the study area. Absence of Van A gene does not rule out that these strains are not VRSA or VISA.
Key words: Molecular characterization, VISA, VRSA, MRSA isolates