Original Research Article
Year: 2019 | Month: April | Volume: 9 | Issue: 4 | Pages: 22-26
Prevalence of Vancomycin Resistant Enterococcus in Various Clinical Specimens in Tertiary Care Hospital
Dilshad Arif1, Atosh Tripathi2, Pratiksha Srivastava2, Satendra Pratap Singh1, Dakshina Bisht3
1Tutor, Department of Microbiology, Government Medical College & Super Facility Hospital, Azamgarh, U.P., India.
2Assistant Professor, Department of Microbiology, Government Medical College & Super Facility Hospital, Azamgarh, U.P., India.
3Prof & HOD, Department of Microbiology, Santosh Medical College, Ghaziabad, NCR, Delhi.
Corresponding Author: Pratiksha Srivastava
ABSTRACT
Introduction - Enterococci form a part of the normal flora of the intestinal tract, oral cavity and the vagina. (1) Vancomycin resistant Enterococci (VRE) have caused hospital acquired infections, outbreaks worldwide which have been dramatically amplified in recent years. Most common infections caused by Enterococci are endocarditis, bacteraemia, intra abdominal and urinary tract infections etc.
Aim & Objective - To determine the prevalence and the Antibiotic Susceptibility pattern of Enterococci isolated from various clinical specimens & To determine the MIC of Vancomycin resistant Enterococci.
Material and Method - The present study was conducted in the Department of Microbiology, Government Medical College, Azamgarh, UP, India. The samples which included were urine, pus, blood wound swab, throat swab and other body fluids. Presumptive identification of Enterococcus was done on the basis of colony characteristics, Gram’s staining & Catalase test. Confirmation was done by growth in 6.5% NaCl, & Bile esculin hydrolysis. Antibiotic Susceptibility Testing done by Kirby Bauer Disc Diffusion Method. MIC was done by the E test for all the Enterococci isolates which showed resistance Vancomycin by the Kirby Bauer disc diffusion method.
Result - A total of 53 isolates of Enterococcus were obtained from various clinical samples. Out of 53, 32 (60.3%) were Enterococcus faecalis and 21 (39.6%) were Enterococcus faecium. Among 53 isolated Enterococcus, 16 isolates (30%) were Vancomycin resistant. Conclusion - This study demonstrates the increased prevalence of multidrug resistant Enterococci, thus posing a serious therapeutic challenge.
Key words: E-Test, Vancomycin resistant Enterococcus, MIC, Bile esculin