Original Research Article
Year: 2019 | Month: February | Volume: 9 | Issue: 2 | Pages: 68-76
Efficacy of Tulsi (Ocimum sanctum) Extract Incorporated onto Guided Tissue Regeneration (GTR) Membrane against Periodontal Pathogens
Dr. Devang Bharatkumar Khambholja1, Kinjal Bharatbhai Mehta2
1Assistant Professor, 2Post Graduate Students,
B.N. Patel Institute of Paramedical and Science (Paramedical Division), Near N.S. Patel Circle,
Anand - 388001, Gujarat, India.
Corresponding Author: Dr. Devang Bharatkumar Khambholja
ABSTRACT
Periodontitis is one of most common oral disease after dental caries in developing countries. The core part of periodontal treatment is to prevent further development of disease in order to restore the damaged tissues. Guided Tissue Regeneration (GTR) membrane has been shown to be a successful treatment modality; however, bacterial contamination on GTR membrane is the major associated concern. Thus, the present study was designed to increase treatment modality by embedding natural antibacterial agent (Tulsi extract) onto GTR membrane. A subgingival microbial sample of chronic periodontitis patient was collected and processed for bacterial isolation on ciprofloxacin embedded plates. Out of total 25 bacterial isolates, CDK-6 and CDK-13 showed maximum ciprofloxacin tolerance were selected for further studies. The 16S rRNA ribotyping identifies strains CDK-6 and CDK-13 as Streptococcus sp. and CDK-13 as Rhodococcus sp. respectively. Results showed that 50µg/ml Tulsi extract was competent to inhibit the complete growth of both the bacterial isolates. Suggesting, the sterile conditions in the periodontal pockets after phase-I therapy of Periodontitis can be maintained by coating GTR membrane with Tulsi extract.
Key words: Tulsi, Periodontitis, GTR membrane, Streptococcus sp., Rhodococcus sp.