Original Research Article
Year: 2019 | Month: December | Volume: 9 | Issue: 12 | Pages: 49-53
Study the Anti-Bacterial Activity of Homoeopathic Medicines against Staphylococcus epidermidis in-vitro
Aishwarya Dilip Pasalkar1, Suyash Arunrao Kathade2, Arun Bhargav Jadhav3, Bipinraj Nirichan Kunchiraman4, Chetan Hanamantrao Shinde5
1Post-Graduate Scholar, 5Associate Professor,
Department of Homoeopathic Pharmacy, Bharati Vidyapeeth (Deemed to be University), Homoeopathic Medical College & Hospital, Katraj – Dhankawadi, Pune, India.
2Research Scholar, Department of Microbial Biotechnology, Bharati Vidyapeeth (Deemed to be University), Rajiv Gandhi Institute of Information Technology and Biotechnology, Katraj – Dhankawadi, Pune, India.
3Principal, Bharati Vidyapeeth (Deemed to be University), Homoeopathic Medical College and Hospital, Katraj - Dhankawadi, Pune, India.
4Assistant Professor, Department of Microbiology, Bharati Vidyapeeth (Deemed to be University), Rajiv Gandhi Institute of Information Technology and Biotechnology, Katraj – Dhankawadi, Pune, India.
Corresponding Author: Aishwarya Dilip Pasalkar
ABSTRACT
The Staphylococcus epidermidis is a facultative anaerobic gram-positive bacterium and is a part of normal human flora which most commonly causes diseases like prosthetic valve disease, endocarditis, and hospital acquired infections particularly seen in people with catheter or other surgical implants. This study is aimed to screen Homoeopathic Medicines such as Antimonium crudum, Arsenic album, Hepar sulphur, Silicea, and Kali bichromicum with potencies of 6C, 12C,30C, 200C, 1M, 10M.for antibacterial activity against Staphylococcus epidermidis. Medicines were screened by Agar well diffusion method, MIC assay and Bactericidal activity. Where in Arsenic album 10M (1.06 ± 0.05)cm, Hepar Sulphur 30C (1.1 ±0.1)cm, 200C (1.03±0.20)cm, 10M (1±0.26)cm, Silicea 10M (0.93±0.28)cm, 1M (0.9±0.17)cm, Kali bichromicum 1M (1.06±0.25)cm shows the Zone of inhibition in Agar well diffusion assay. Where in MIC assay in each potency of four Homoeopathic Medicines among the five Homoeopathic Medicines such as in Arsenic album 6C, Antimonium crudum 200C, Hepar sulphur 200C, Silicea 12C.In Bactericidal study there were more dead cells as compared to live cells.
Key words: Anti-bacterial, Homeopathic Medicines, In-vitro, Staphylococcus epidermidis