Original Research Article
Year: 2019 | Month: December | Volume: 9 | Issue: 12 | Pages: 39-43
An in-vitro Study to Evaluate the Anti-Bacterial Activity of Rauvolfia serpentina against Escherichia coli
Anupam Mukherjee1, Mayur Arjun Aswani2, Arun Bhargav Jadhav3, Bipinraj Nirichan Kunchiraman4 and Chetan Hanamantrao Shinde5
1Post Graduate Scholar, 5Associate Professor,
Department of Homoeopathic Pharmacy, Bharati Vidyapeeth (Deemed to be University) - Homoeopathic Medical College & Hospital, Katraj-Dhankawadi, Pune, Maharashtra, India.
2Research Scholar, Department of Herbal Medicine, Bharati Vidyapeeth (Deemed to be University) - Interactive Research School for Health Affairs, Katraj - Dhankawadi, Pune, India.
3Principal, Bharati Vidyapeeth (Deemed to be University) - Homoeopathic Medical College & Hospital, Katraj - Dhankawadi, Pune, Maharashtra, India.
4Assistant Professor, Department of Microbiology, Bharati Vidyapeeth (Deemed to be University) -Rajiv Gandhi Institute of Information Technology and Biotechnology, Katraj-Dhankawadi, Pune, Maharashtra, India.
Corresponding Author: Anupam Mukherjee
ABSTRACT
World Health Organization’s recent Global Antimicrobial Surveillance System (GLASS) reveals widespread occurrence of antibiotic resistance, among which the most commonly reported resistant bacteria, is Escherichia coli. Due to the unethical use of antibiotics, apart from drug resistance, generation of new mutant forms of non-pathogenic bacteria is also posing a severe threat to medical science nowadays. E. coli is a gram-negative, facultatively anaerobic bacterium which is commonly found in the intestine. This bacterium can cause various diseases such as Urinary Tract Infection (uropathogenic E. coli), Gastroenteritis, and Neonatal meningitis. In rare instances, they are also responsible for Septicaemia, Mastitis, Haemolytic-Uremic Syndrome (HUS) and Gram-negative Pneumonia. This study is aimed to evaluate the antibacterial activities of homoeopathic medicine Rauvolfia serpentina against E. coli. Thus it is used in various potencies to screen the bacteria by Agar Well-Diffusion assay, MIC assay and Bactericidal study. Rauvolfia serpentina 6C (0.9±0.1) c.m., 30C (0.6±0.1) c.m., 200C (0.7±0.1) c.m., 1M (0.8±0.1) c.m. showed inhibitory activity against E. coli by Agar well-diffusion assay. Rauvolfia serpentina in 6C showed maximum Growth inhibitory zone (GIZ) against E. coli in optical density value at 600 nm by MIC assay after comparison with Positive control (Amikacin), Vehicle control (Dispensing alcohol or ethanol 90%), Culture control (broth + culture), Negative control (Mueller Hinton Broth). Also in Bactericidal study with Rauvolfia serpentina 6C, maximum dead cells have been seen as compare to the live cells in the death phase.
Key words: Anti-bacterial activity, Escherichia coli, Minimum Inhibitory Concentration, Bacteriolytic study, Rauvolfia serpentina, Homoeopathy.