Original Research Article
Year: 2018 | Month: November | Volume: 8 | Issue: 11 | Pages: 88-96
Molecular Detection of Carbapenem Resistant Klebsiella pneumoniae in Intensive Care Units
Archana Hegde M1, Tejashree A2, Shahnawaj Mehdi3
1Research Scholar, 2Professor, 3Post Graduate,
Department of Microbiology, JSS Medical College, Mysuru.
Corresponding Author: Tejashree A
ABSTRACT
Carbapenem Resistant Klebsiella pneumoniae carrying blaKPC, blaNDM-1 and blaOXA-48 genes are difficult to treat. This study was conducted to study the genotypic features of carbapenem resistance in isolates of Klebsiella pneumoniae isolated from Intensive care units from a tertiary care Hospital. Klebsiella pneumoniae (n=89) isolates resistant to at least one carbapenem were collected from 377 patients for 24 months. Carbapenem resistance was determined via VITEK-2 system and confirmed my Modified Hodge test. Genes were analyzed using Polymerase chain reaction (PCR). The rate of resistance by Vitek-2 system for carbapenems was 23.6% and Modified Hodge test against ertapenem was 83% for 89 CRKP isolates. There were 39%, 29% and 46% of isolates positive for blaKPC, blaNDM-1 and blaOXA-48 genes respectively. The presence of all three genes showed 8.9% positivity. The molecular classification of carbapenemases will be useful for the betterment of patients with concurrent carbapenem resistant Enterobacteriaceae (CRE) infections. This could prevent outbreaks and complications in Intensive care units.
Key words: Klebsiella pneumoniae, Carbapenem resistance, blaKPC, blaNDM-1 and blaOXA-48 gene.