Original Research Article
Year: 2017 | Month: April | Volume: 7 | Issue: 4 | Pages: 132-137
ESBL Producing Pseudomonas Aeruginosa: A Threat to Patient Care
Dr. Sanchari Das1, Dr. Silpi Basak2
1Post Graduate Student, 2Professor,
Department of Microbiology, Jawaharlal Nehru Medical College, Wardha (MS), Pin-442004
Corresponding Author: Dr. Silpi Basak
ABSTRACT
Background- Pseudomonas aeruginosa, well known as opportunistic pathogen, has been implicated in life threatening nosocomial infections in recent years. Resistance of Pseudomonas aeruginosa strains to the oxyimino cephalosporins may be caused by production of extended-spectrum β-lactamases (ESBLs).
Aim and objective- To detect the incidence of ESBL producing Pseudomonas aeruginosa strains isolated from different clinical specimens and to study the antibiotic susceptibility profile of ESBL producing Pseudomonas aeruginosa strains.
Material and methods- Various clinical specimens received in Microbiology laboratory were processed and Pseudomonas aeruginosa was identified as per standard procedure. A total number of 150 clinical isolates of Pseudomonas aeruginosa were included in the study. Antimicrobial susceptibility test was performed for all strains by Kirby – Bauer disc diffusion method as per Clinical Laboratory Standard Institute (CLSI, 2016) Guidelines. All 150 isolates were subjected for ESBL screening test and confirmatory Combined Disc method and E Test.
Observation and results- Out of 150 Ps. aeruginosa strains 90 (60%) were ESBL producers. The highest antibiotic susceptibility was observed with Imipenem (87.8 %) and the maximum 41.4% of ESBL producer strains were isolated from pus and wound swab.
Conclusion- Early detection of ESBL producing Pseudomonas aeruginosa is the need of the hour for effective treatment of patients and prevention of spread of ESBL producing strains in health care set up.
Key words: Pseudomonas aeruginosa, antibiotic susceptibility test, ESBL production, combined disc method, E test.