Original Research Article
Year: 2014 | Month: January | Volume: 4 | Issue: 1 | Pages: 79-84
Acinetobacter Species an Emerging Nosocomial Pathogen: It’s Isolation Pattern, Biofilm Formation and Antibiotic Susceptibility Profile
Anagha Kinikar1*, S. Saini2, Seema Bose3, Sachin Deorukhkar4
1Associate Professor, 2Professor and Head, 3Professor, 4Assistant Professor,
Dept. of Microbiology, Rural Medical College, Pravara Institute of Medical Sciences (DU), Loni - 413736, Maharashtra, India.
*Corresponding Author: Anagha Kinikar
ABSTRACT
Introduction: Bacteria of genus Acinetobacter are aerobic, nonfermentative, Gram negative bacilli that cause wide variety of illnesses. Acinetobacter species isolates are frequently resistant to multiple antibiotic classes through an array of resistant mechanism. Biofilm production by bacteria is associated with chronic nosocomial infection.
Aim and objective: To study the isolation pattern, biofilm formation and antibiotic susceptibility profile of Acinetobacter species isolated from various clinical specimens.
Materials and methods: The study included 166 Acinetobacter species isolates isolated from various clinical specimens. All the isolates were subjected to antibiotic susceptibility using modified Kirby- Bauer disc diffusion method. Detection of biofilm was done by tube adherence method and Congo red agar method.
Result: Out of 166 Acinetobacter species isolates, 70 isolates (42.1%) showed biofilm formation by either method. Biofilm producer Acinetobacter isolates showed significantly higher resistance than non-biofilm producer.
Conclusion: In the present study Acinetobacter species isolates producing biofilm showed high degree of resistance to most of the drugs including carbapenem. Detection of biofilm producer strains can help in appropriate selection of antibiotic. The Acinetobacter species needs to be considered as an essential pathogen and steps must be taken to control such infections.
Key words:Acinetobacter, Biofilm, Congo red method, Tube adherence method, Multi-drug resistance