Original Research Article
Year: 2014 | Month: January | Volume: 4 | Issue: 1 | Pages: 65-68
Longevity of DPX Mounted Slides in Neurohistology
R Victor1#, Yogitha Ravindranath2*@,Roopa Ravindranath2**, V Sumithra2***
#Consultant Cytogenetics,* Associate Professor,** Professor,*** Senior Lab Technician,
1S-DACC, Shreyas Diagnostic, Andrology & Cytogenetics Centre, Bangalore.
2Histology Laboratory, Department of Anatomy, St John’s Medical College, Bangalore– 560034.
@Corresponding Author: Yogitha Ravindranath
ABSTRACT
This study was undertaken to review the following aspect of the mounting medium DPX. The longevity of the medium, preservation of the Neurohistological stains, to observe for formation of air space under the cover glass.
Two sets of slides stained in 1967 and 1987 were selected from the collection of histology laboratory. These slides were well fixed and processed human central nervous system paraffin blocks of six micron thickness.
The first set of slides were stained with various histological stains i.e. Margolis, Kluver Berrera, Luxol fast blue, Cresyl fast violet, Mallory Azan and mounted in DPX mountant in 1967.
The second set of slides was stained with Catechu stain, which was developed and mounted in the DPX mountant in 1987 by one of the authors.
These set of slides have been carefully monitored from time to time for any deterioration of structures, preservation, fading of stains and development of air spaces for the last 45 years. Till recent years these slides were used as demonstration slides during neurohistology practicals.
On observation it has been found that the DPX mountant has not caused any deterioration what so ever and the structures are well appreciated, as though the staining has been performed recently. It appears that the DPX mountant will preserve the stained slides for a few more decades without deterioration.
Key words:central nervous system, paraffin sections, staining, DPX mountant