Year: 2024 | Month: May | Volume: 14 | Issue: 5 | Pages: 160-167
DOI: https://doi.org/10.52403/ijhsr.20240518
The Cell Viability of Dental Materials by The Lactate Dehydrogenase Assay
Hakan Kamalak
Department of Restorative Dentistry, Kahramanmaras Sutcu Imam, Faculty of Dentistry, Onikisubat, Turkey.
ABSTRACT
Objectives: the objective of this study is to search the cell membrane damage of resin composite materials on cultures of human gingival fibroblasts and neuron cells by the lactate dehydrogenase (LDH) test method.
Methods: A range of thirteen resin composites sterilized under UV light for 2 hours were added to the culture medium of human gingival fibroblasts and neuron for 24 h and 72h. The samples were moved to 48- well-plates in a sterile cabin, one by one, in direct contact with the cells. After 24- and 72-hours incubation period, in the incubator at 37 oC with 5% CO2; Cell membrane damage was detected by the lactate dehydrogenase (LDH) test method. Results: In the lactate dehydrogenase (LDH) test method, cytotoxicity is determined by measuring lactate dehydrogenase, which is a cytosolic enzyme released in the event of membrane damage or cytolysis in the cell. A higher LDH value indicates that the toxicity of the material is greater. When the LDH results in Gingival fibroblast cells were examined after 24 hours, F25 was found to be the most toxic, and when the LDH results in neuron cells were examined, ISM and F25 were also found to be the most toxic.
Significance: Dental restorative materials have some toxic effects on cell cultures. The LDH analysis supplies precious instructions about their toxic effects.
Key words: Lactate dehydrogenase, fibroblasts, neuron cells, cytotoxicity