Year: 2024 | Month: February | Volume: 14 | Issue: 2 | Pages: 76-81
DOI: https://doi.org/10.52403/ijhsr.20240210
Association of IFN-γ, IL-4 and Interleukin-10 Gene Polymorphisms with Preeclampsia
Komal Uppal1, Madhavi Puppala2, Lakshay Rana3, Sunil Kumar Polipalli4, Lakshmi Kalpana Veerathu5
1,3Paediatrician, 2Research Scholar, 4Scientiest, 5Professor
1,3,4Division of Genetics and Metabolism, Department of Pediatrics, Maulana Azad Medical College (Delhi University), Delhi 110002.
2,5Dept. of Human Genetics, Andhra University, Visakhapatnam, Andhra Pradesh, 530003.
Corresponding Author: Sunil Kumar Polipalli
ABSTRACT
Background: In view of significant effect of cytokine-polymorphisms on inflammation and immune regulation, and Inflammatory cytokines causing Preeclampsia (PE), it becomes mandatory to examine the role of cytokine-SNPs in all pathological mechanisms responsible for PE. In this review, we describe association between polymorphisms in IFN-γ (+874 A/T; rs2430561), IL-4, IL-10 (-819), IL-10 (-1082), IL-10 (-592) genes and preeclampsia and healthy controls in North Coastal Andhra Pradesh of India.
Methods: 200 samples (100 preeclamptic and 100 normal pregnant women as control group) were sequenced using allele-specific oligonucleotides-polymerase chain reaction and PCR-RFLP. Data was analyzed using chi-square and Fisher’s exact tests.
Results: IL - 4 (C590T) C/T genotype (odds ratio 2.452, 95% confidence interval (1.299-4.626); P=.002) and genotype CC of IL-10 (C819T) (odds ratio 0.4361, 95% confidence interval (0.2377-0.8001); P=.003) showed a significantly higher frequency among the preeclamptic group than the control group.
Conclusion: Genotype CT of IL-4 (C590T) and genotype CC of IL-10 (C819T) have a significant role in the development of preeclampsia; differing in `genetic predisposition/pathophysiology.
Key words: IFN-γ, IL-4, IL-10, Interleukin-10, Gene Polymorphism, Preeclampsia.