Original Research Article
Year: 2021 | Month: July | Volume: 11 | Issue: 7 | Pages: 89-98
DOI: https://doi.org/10.52403/ijhsr.20210713
Increased Serum Level of 8-Hydroxy-2’-Deoxyguanosine may be an Indication of Pesticides Induced DNA Damage among Indian Farmers
Arun Pandiyan1, Summaiya Lari2, Sudip Ghosh3, Naveen Kumar Boiroju4, Janardhan Vanka5, Yogeswar Dayal Kasa6, Babban Jee7, Padmaja Jonnalagadda8
1,2,6 Senior Research Fellow (ICMR-National Institute of Nutrition, Hyderabad, Telangana, India)
3,8Scientist - F (ICMR-National Institute of Nutrition, Hyderabad, Telangana, India)
4Scientist - C (ICMR-National Institute of Nutrition, Hyderabad, Telangana, India)
5Technical Officer (ICMR-National Institute of Nutrition, Hyderabad, Telangana, India)
7Scientist - C (Department of Health Research, MoHFW, New Delhi, India)
Corresponding Author: Padmaja Jonnalagadda
ABSTRACT
The aim of the present hospital based study is to assess the serum levels of 8-hydroxy-2’-deoxyguanosine (8-OHdG) among 152 subjects. A total of 152 subjects, categorized into 3 groups: (i) pesticides exposed group (N=60), (ii) pesticide un-exposed group (N=42) and (iii) healthy controls group (N=50) were recruited for the study following the inclusion and exclusion criteria. The blood was drawn from the eligible subjects and Enzyme Immuno Assay (EIA) was performed to assess the 8-OHdG levels in serum. Appropriate statistical methods were used to analyse the study data. Assessment showed that pesticides exposed group has higher levels of serum 8-OHdG as compared to un-exposed group and healthy controls. Age and duration of exposure had an impact on the levels of serum 8-OHdG.The higher serum levels of 8-OHdG may be a marker for pesticides-induced oxidative DNA damage.
Key words: pesticides; exposure; oxidative stress; 8-hydroxy-2’-deoxyguanosine (8-OHdG).