Original Research Article
Year: 2021 | Month: February | Volume: 11 | Issue: 2 | Pages: 1-7
Molecular Detection of Association of Vascular Endothelial Growth Factor (VEGF) Gene in Oral Sub Mucosal Fibrosis (OSF) Cancer
Gurushantappa S Kadakol1, Manish Kumar Sahoo1, Vikas C Desai2, Rudragouda S Bulagouda1
1Human Genetics Laboratory Dept. of Anatomy, BLDE (DU) Shri B M Patil Medical College, Hospital & RC Vijayapur, Karnataka, India.
2Department of Dental BLDE (DU) Shri B M Patil Medical College, Hospital & RC Vijayapur, Karnataka, India.
Corresponding Author: Gurushantappa S Kadakol
ABSTRACT
Oral submucous fibrosis (OSMF or OSF) is a chronic, complex, premalignant (1% transformation risk) condition of the oral cavity, characterized by juxta-epithelial inflammatory reaction and progressive fibrosis of the sub mucosal tissues. A recent study shows strong association of VEGF gene in Oral Sub mucosal Fibrosis (OSF) Cancer. To know the association of VEGF gene polymorphism in patients with OSMF and to compare the same among healthy subjects. The study included a total of 50 subjects from patients reporting to the Out-patient Department of Dentistry, ENT of which 30 were diagnosed to have Oral submucous fibrosis and 20 were healthy controls (without habits and free from any lesions). Isolated deoxyribonucleic acid (DNA) samples from both were subjected polymerase chain reaction- based restriction analysis was carried out for VEGF gene. VEGF gene has to be used as a specific biomarker for the Oral submucous fibrosis Cancer (OSMF or OSF).
Key words: Oral submucous fibrosis Cancer (OSMF or OSF); Polymerase Chain Reaction; VEGF Gene.