Original Research Article
Year: 2020 | Month: June | Volume: 10 | Issue: 6 | Pages: 338-345
Detection of Antibiotic Susceptibility Pattern and Methicillin Resistance among the Clinical Isolates of Coagulase Negative Staphylococci (CONS) in a Rural Tertiary Care Hospital
Abhishek Debnath1, Dipayan Ghosh2, Reshmi Ghosh3
1MBBS, MD (Microbiology), Government Medical Officer, Tripura Health Service, Dhaleswar, Agartala, Tripura
2State Mission Manager, Tripura Rural Livelihood Mission, A.D.Nagar, S.D.Mission, Agartala, Tripura
3Research Scholar, Tripura University, Dhaleswar, Agartala, Tripura
Corresponding Author: Abhishek Debnath
ABSTRACT
Introduction: CONS are part of the normal skin flora increasingly recognized as significant nosocomial pathogens and often causes different infections associated with implanted devices, joint prosthesis and different indwelling devices. They are very difficult to treat as they are more resistant to commonly used antimicrobial agents than others. Because of that, present study was conducted with following aims and objectives.
Aims and Objectives: To study antibiotic susceptibility profile of isolated species of CONS to various classes of antimicrobials using Kirby Bauer disc diffusion method followed by detection of methicillin resistance and inducible clindamycin resistance among the isolated species of CONS.
Materials and Methods: 170 CONS strains isolated from clinically significant samples were identified by different conventional methods and antibiotic resistance pattern was detected by Kirby-Bauer Disk Diffusion method by using different antibiotic discs. Methicillin resistance and Inducible and constitutive clindamycin resistance (D test) was detected according to CLSI guidelines.
Results: Among 170 CONS isolates, predominant isolated species were S. epidermidis (42.35%), S.haemolyticus (27.06%) and showed higher resistance to different antibiotics. 105(61.76%) isolates showed erythromycin resistance, out of which, 26 (24.76%) isolates were iMLSB. 113(66.47%) isolates were MRCONS.
Conclusion: Present study showed high prevalence of MRCONS, resistant to widely used antimicrobial agents. Hence, it is necessary to have regular surveillance of MRCONS which will be useful for selecting an appropriate antibiotic.
Key words: Coagulase negative staphylococci (CONS), inducible clindamycin resistance (iMLSB)