Original Research Article
Year: 2020 | Month: June | Volume: 10 | Issue: 6 | Pages: 12-18
Detection of Inducible Clindamycin Resistance (iMLSB) among the Erythromycin Resistant CONS Isolates in a Rural Tertiary Care Hospital- Need of Time
Abhishek Debnath1, Reshmi Ghosh2, Dipayan Ghosh3
1Dept. of Microbiology, Jawaharlal Nehru Medical College, Wardha, Maharashtra
2Research Scholar, Tripura University, Dhaleswar, Agartala, Tripura
3State Mission Manager, Tripura Rural Livelihood Mission, A.D.Nagar, S.D.Mission, Agartala, Tripura
Corresponding Author: Abhishek Debnath
ABSTRACT
Introduction: Therapeutic failure of clindamycin has been reported due to various mechanisms that confer resistance to macrolide, lincosamide and streptogramin antibiotics. Because of that, present study was conducted with following aims and objectives.
Aims and Objectives: To detect inducible clindamycin resistance among the erythromycin resistant CONS isolates and correlation with methicillin resistance.
Materials and Methods: 180 CONS strains isolated from clinically significant samples were identified by different conventional methods and Erythromycin resistance was detected by Kirby-Bauer Disk Diffusion method. Methicillin resistance and Inducible and constitutive clindamycin resistance (D test) was detected according to CLSI guidelines.
Results: Among 180 CONS isolates, predominant isolated species were S. epidermidis 75(41.67%), S.haemolyticus 47(26.11%). Out of 180 CONS isolates, 108(60%) showed erythromycin resistance, out of which, 29 (26.85%) isolates showed iMLSB. Among 180 CONS isolates, 119(66.11%) were MRCONS isolates and 61(33.89%) were MSCONS isolates.
Conclusion: D test for detection of inducible clindamycin resistance should be included in the routine clinical laboratories as part of antibiotic susceptibility testing, as it will help physicians in guiding therapy.
Key words: Coagulase negative staphylococci (CONS), inducible clindamycin resistance (iMLSB)