Original Research Article
Year: 2020 | Month: January | Volume: 10 | Issue: 1 | Pages: 201-205
The Effect of Intermittent Negative Pressure on the Amputation Stump of Adult Mice: A Pilot Study
Osama Sharafeldin Mohammedahmed Abbadi1, Khalid Mohamed Nafie2
1Lecturer of Biochemistry, Faculty of Medicine, Omdurman Islamic University, Sudan.
2Consultant of Pathology and Laboratory Medicine, Ministry of Health, Saudi Arabia, Prince Mishari bin Saud Hospital, Saudi Arabia.
Corresponding Author: Osama Sharafeldin Mohammedahmed Abbadi
ABSTRACT
Overview: Limb amputation could occur accidently or due to an inevitable medical indication such as cancer, vascular damage, or traumatic destruction. Whatever the cause, a lost limb does not regenerate in mammals and most animals; however, studies showed that young mice have an ability to restore a distally amputated finger tip. There are evidences the in literature supporting that applying intermittent negative pressure to tissues could trigger the release of growth factors; which are important for tissue regeneration.
Objectives: To document the effects of the intermittent negative pressure-INP in an amputation stump of albino mice, particularly if there are evidence of cellular proliferation or tissue remodeling.
Methods: Two albino mice, three weeks of age, were first cut at the wrist level on their right arms. One mouse was subjected to manual INP by a fifty milliliter pump syringe, ten minutes daily for three weeks, the other mouse left to heal spontaneously, as a control.
Results: There was an excessive fibrous tissue formation within the stump, seen by the naked eye. Microscopic examination showed thinning of the epithelial lining, areas of fluid and inflammatory cells collection, and excessive fibrous tissue formation.
Conclusion: Negative pressure does not enhance the growth or regeneration of amputated limbs in adult mice; instead, it caused an inflammatory-like response and fibrous tissue formation.
Key words: Amputation, Negative pressure, Albino mice.